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- M. Balachander
- G. Roopa
- R. Rangeshwaran
- Pritam Karmakar
- S. Sriram
- M. J. Savitha
- Karmakar Pritam
- N. V. Vajid
- T. V. Bhaskaran
- Satendar Kumar
- Vinaya Hemannavar
- Honnur Basha
- R. Nirmala
- R. J. Rabindra
- N. S. Rao
- A. Derakhshan
- C. Gopalakrishnan
- R. D. Prasad
- V. K. Razdan
- S. Renuka
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- Krishna Japur
- Rudra N. Borkakati
- D. K. Saikia
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Ramanujam, B.
- ITS Sequencing of Indian Isolates of Lecanicillium Species
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Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, HA Farm Post, Bellary Road, Bangalore 560 024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, HA Farm Post, Bellary Road, Bangalore 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 25, No 4 (2011), Pagination: 337-341Abstract
The genetic diversity of thirty one isolates of Lecanicillium species isolated from insect hosts from various geographical regions of India were studied. Their phylogenetic relationships were determined using internal transcribed spacer, ITS1, ITS2 and 5.8S gene of rRNA sequence. Based on the sequence similarity of ITS region and construction of subsequent phylogenetic analysis (neighbour-joining method), 15 isolates were grouped as Lecanicillium lecanii, 11 isolates as L. attenuatum, 3 isolates as L. longisporum and 2 isolates as L. muscarium.Keywords
ITS Region, Phylogenetic Analysis, Lecanicillium sp.Full Text
References
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- Carder, J. H. and Barbara, D. J. 1999. Taxonomic status of putative Verticillium albo-atrum isolates. FEMS Microbiology Letters, 170: 211–219.
- Gams, W. and Zare, R. 2001. A revision of Verticillium sect. Prostrata. III. Generic classification. Nova Hedwigia, 72: 47–55.
- Hall, R. A. 1981. Laboratory studies on the effects of fungicides, acaricides and insecticides on the entomopathogenic fungus, Verticillium lecanii. Entomologia Experimentalis et Applicata, 29: 39–48.
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- Sugimoto, M., Koike, M., Hiyama. N. and Nagao, H. 2003. Genetic, morphological and virulence characterization of the entomopathogenic fungus Verticillium lecanii. Journal of Invertebrate Pathology, 82: 176–187.
- Sung, G. H., Zare, R., Spatafora, J. and Gams, W. 2001. Verticillium section Prostrata. 11. Generic delimitation using ribosomal DNA sequences. Nova Hedwigia, 72: 29–46.
- Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positionspecific gap penalties and weight matrix choice. Nucleic Acids Research, 22: 4673–4680.
- White, T. J., Bruns, T. D., Lee, S. B. and Taylor, J. W. 1990. Amplication and direct sequencing of fungal ribosomal RNA genes for phylogenetics. pp. 315–322. In: Innis M.A., Gelgand, D.H., Suinsky, J.J. and White, J.J., (Eds.), PCR protocols: A Guide to Methods and Applications, Academic Press, San Diego.
- Zare, R. and Gams, W. 2001. A revision of Verticillium section Prostrata. IV. The genera Lecanicillium and Simplicillium gen. nov. Nova Hedwigia, 73: 1–50.
- Zare, R. and Gams, W. 2004. A monograph of Verticillium section Prostata. Rostaniha, 3: 1–188.
- Zare, R., Gams, W. and Culham, A. 2000. A revision of Verticillium sect. Prostrata. 1. Phylogenetic studies using ITS sequences. Nova Hedwigia, 71: 465–480.
- Zare, R., Gams. W. and Evans, H. C. 2001. A revision of Verticillium section Prostrata. V. The genus Pochonia, with notes on Rotiferophthora. Nova Hedwigia, 73: 51–86.
- Trichoderma-enriched Coco-Peat for the Management of Phytophthora and Fusarium Diseases of Chilli and Tomato in Nurseries
Abstract Views :314 |
PDF Views:190
Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, H. A. Farm Post, Hebbal, Bellary Road, Hebbal, Bangalore, 560 024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, H. A. Farm Post, Hebbal, Bellary Road, Hebbal, Bangalore, 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 24, No 4 (2010), Pagination: 311-316Abstract
Coconut coir dust, commercially available as coco-peat, is used in raising the seedlings of vegetable crops in tropical countries. Coir-pith and other derivates of coconut husk have been well recognized as substrates for the multiplication of Trichoderma spp. and commercial nurseries use coco-peat for raising the seedlings. In the present study, coco-peat enriched with Trichoderma harzianum was used for raising tomato and chilli seedlings to test the effect of the same on managing wilt caused by Fusarium oxysporum f. sp. lycopersici in tomato and damping off and ischolar_main rot caused by Phytophthora capsici in chillies in nurseries. The enrichment with T. harzianum, resulted in reduced wilt incidence (5-7.5%) compared to control (38.75%) in tomato with increased plant growth parameters. Though germination was reduced compared to control (without pathogen), there was reduction in P. capsici infection in chillies by up to 50% compared to coco-peat without Trichoderma enrichment. The use of coco-peat enriched with T. harzianum can be adopted by commercial nurseries for better plant growth and reduced incidence of tomato wilt and chilli ischolar_main rot while raising disease free and healthy seedlings.Keywords
Trichoderma spp., Coco-Peat, Fusarium oxysproum f. sp. lycopersici, Phytophthora capsici, Tomato, Chilli, Commercial Nurseries.Full Text
- Chitinase Activity and Virulence of Different Isolates of Beauveria bassiana, Metarhizium anisopliae and Lecanicillium Spp
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Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 25, No 3 (2011), Pagination: 223-228Abstract
In order to identify promising isolates with higher chitinase activity, 122 entomofungal isolates comprising of Beauveria bassiana (58), Metarhizium anisopliae (33), Lecanicillium lecanii (15), Lecanicillium attenuatum (11), L. longisporum (3) and L. muscarium (2) isolated from different insect hosts/soils of India were studied. The partially purified proteins from the isolates were subjected to chitinase activity and were estimated by measuring the release of reducing saccharides (one NAGA Unit) from colloidal chitin spectrophotometrically at 582 nm (A582). Forty nine isolates of B. bassiana showed chitinase activity ranging from 21 to 182 μg/ml, with the highest enzyme activity by the isolate PDBC-Bb-5a. Thirty three isolates of M. anisopliae exhibited chitinase activity ranging from 23 to 144 μg/ml and the highest (144 μg/ml) was by the isolate Ma-4. Among the 15 isolates of L. lecanii tested, three isolates viz., Vl-7, Vl-24a, Vl-25a had high chitinase activities ranging between 100 and 126μg/ml. Vl-22 isolate of L. attenuatum, Vl-24 of L. longisporum and Vl-8 of L. muscarium showed higher activities (90, 110 and 117μg/ml respectively). Bioassay studies with these isolates on cowpea aphid, Aphis craccivora in glass house indicated higher nymphal mycosis ranging from 72.3-83.0%.Keywords
Entomopathogenic Fungi, Chitinase Activity, NAGA Unit, Cowpea Aphid, Aphis craccivora, Virulence.Full Text
References
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- Havukkala, I., Mitamura, C., Hara, S., Hirayae, K., Nishizawa, Y. and Hibi, T. 1993. Induction and purification of Beauveria bassiana chitinolytic enzymes. Journal Invertebrate Pathology, 61: 97– 102.
- Hepburn, H. R. 1985. Structure of the integument. In Kerbut, G.A. and Gilbert, L.I, (eds) Comprehensive Insect Physiology, Biochemistry and Pharmacology. Volume, 3, pp. 1–58. Pergamon Press, Oxford, UK. Krieger de Moraes, C., Schrank, A. and Vainstein, M. H., 2003. Regulation of extracellular chitinase and proteases in the entomopathogen and acaricide Metarhizium anisopliae. Current Microbiology, 46: 205–210.
- Nahar, P., Ghormade, V. and Deshpande, M.V. 2004. The extracellular constitutive production of chitin deacetylase in Metarhizium anisopliae: Possible edge to entomopathogenic fungi in the biological control of insect pests. Journal of Invertebrate Pathology, 85: 80–88
- Samuels, K. D. Z., Heale, J. B. and Llewellyn, M. 1989. Characteristics relating to the pathogenicity of Metarhizium anisopliae toward Nilaparvata lugens. Journal of Invertebrate Pathology, 53: 25–31.
- St. Leger, R. J., Cooper, R. M. and Charnley, A. K. 1991. Characterization of chitinase and chitobiase produced by the entomopathogenic fungus Metarhizium anisopliae. Journal of Invertebrate Pathology, 58:415–426.
- Yanai, K., Takaya, N., Kojima, M., Horiuchi, H., Ohta, A. and Takaki, M. 1992. Purication of two chitinases from Rhizopus oligosporus and isolation and sequencing of the encoding genes. Journal of Bacteriology, 174: 7398–7406.
- Additives in Powder Based formulation for Enhanced Shelf Life of Pseudomonas fluorescens and Bacillus sp.
Abstract Views :286 |
PDF Views:146
Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Hebbal, Bellary Road, Bangalore 560 024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Hebbal, Bellary Road, Bangalore 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 24, No 2 (2010), Pagination: 158-163Abstract
Studies were carried out to evaluate the effect of adding nutrient additives to talc based formulations on the shelf life of bacteria stored under room temperature. Two organisms namely, Pseudomonas fluorescens (PDBCAB2) and Bacillus sp. (MTCC6534 - chickpea endophyte), a spore forming bacterium, were tested. A gradual increase in P. fluorescens population up to 90 days was observed in almost all the treatments that were amended with nutrients. At 90 days highest population of log 10.38 cfu g−1 was noticed in talc amended with 2 per cent tryptone and glycerol. Decline in the population was observed from 90 days onwards and was rapid from 150 days onwards. At 240 days highest count of log 1 x 104.2 cfu g−1 was obtained with formulations amended with 2% tryptone and 2% glycerol and the results indicated that 2% peptone or 2% tryptone supplemented with 2% glycerol helped P. fluorescens in its better survival. The spore forming Bacillus sp. survived well throughout the study period. At 240 days all talc formulations amended with nutrient sources showed a population of log 9.0 cfu g−1 or above and the population declined to below log 7.0 cfu g−1 only in non amended treatments. Slow decline started from 240 days but high cfu of log 9.30 to 9.38 g−1 were noticed in yeast extract or tryptone treated treatments. Hence talc formulations that had yeast extract or tryptone supplemented with glycerol enhanced shelf life of Bacillus spp.Keywords
Pseudomonas fluorescens, Bacillus sp, Powder Based Formulation, Talc, Shelf Life, Gram Positive, Gram Negative.Full Text
- Post Harvest Fruit Bioassay of Phylloplane, Pomoplane and Endophytic Microbes against Chilli Anthracnose Pathogen, Colletotrichum capsici (Syd.) E. J. Butler&bisby
Abstract Views :246 |
PDF Views:141
Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, HA Farm post, Bellary Road, Hebbal, Bangalore 560024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, HA Farm post, Bellary Road, Hebbal, Bangalore 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 26, No 1 (2012), Pagination: 62-69Abstract
Two hundred and fifty eight phylloplane/endophytic bacterial isolated from chilli leaves/fruits and one hundred pomoplane yeast isolates from vegetable/fruits were screened against Colletotrichum capsici by fruit bioassay (post harvest) method. Among the pomoplane bacterial isolates tested, Bacillus tequilensis (PMB-185) gave highest reduction (67.84%) of lesion development, where as among the phylloplane bacterial isolates, PHB-25 exhibited highest (48.65%) suppression of lesion caused by C. capsici. Among the endophytes tested, B. megaterium (ENB-86) produced the highest suppression of lesion (59.66%) and rhizospheric bacterium Pseudomonas putida (PBA-5) showed 50.68% suppression. Six bacteria exhibiting significant suppression (50.29 to 67.84%) were identified by 16s rDNA analysis and all of them belonged to Bacillus spp. including B. tequilensis (PMB-185), B. pumilus (PMB- 183), two B. subtilis (PMB-123 and ENB-24) and two B. megaterium (PMB-53 and ENB-86). Among the yeast isolates tested, the maximum reduction (72.16%) of lesion development was observed with the yeast isolate, Hanseniaspora uvarum (Y-73) which was the highest among all the antagonists tested. The results indicated that spraying of H. uvarum (Y-73) or B. tequilensis (PMB-185) on freshly harvested chilli fruits reduced post harvest fruit damage by C. capsici in chilli.Keywords
Chilli Anthracnose, Colletotrichum capsici, Fruit Bioassay, Pichia guilliermondii and Bacillus Species.Full Text
References
- Chanchaichaovivat A, Ruenwongsa P, Panijpan B. 2007. Screening and identification of yeast strains from fruits and vegetables: Potential for biological control of postharvest chilli anthracnose (Colletotrichum capsici). Biol Control. 42: 326–335.
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- Xue-qing CAI, Hong HE, Yi-yuan YE, Fang-ping HU. 2004.Effect of endophytic bacteria BS-1 and BS-2 (Bacillus subtilis) on the control of capsicum anthrancnose and active oxygen metabolism in capsicum fruits. J Fug Agri Uni. 1: 102–105.
- Effect of Entomofungal Pathogens on Mortality of Three Aphid Species
Abstract Views :203 |
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Authors
Affiliations
1 Project Directorate of Biological Control (leAR) Post Bag No. 2491, H. A. Farm Post, Bellary Road Hebbal, Bangalore 560024, Karnataka, IN
1 Project Directorate of Biological Control (leAR) Post Bag No. 2491, H. A. Farm Post, Bellary Road Hebbal, Bangalore 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 20, No 1 (2006), Pagination: 89-94Abstract
The pathogenicity of twelve fungal isolates belonging to Beauveria basiana (Bals.) Vuill., Metarhizium allisopliae (Metschinikoff) Sorokin and Verticillium lecanii (Zimmerman) against Aphis craccivora Koch, Aphis gossypii Glover and Rhopalosiphum maidis Fitch was studied using detached leaf bioassay technique. All twelve isolates of thc three fungi were found to be pathogenic to A. craccivora and A. gossypii at a concentration of 1x107 spores/ml. All isolates except Bb3 and Bb4 of B. bassiana were pathogenic to R. maidis. The mortality ranged from 2 to 74 per cent in A. craccivora, 14 to 80.8 percent in A. gossypii and 6 to 50 per cent in R. maidis. Bb5a isolate of B. bassiana caused highest per cent mortality in A. gossypii (80.8%.) and R. maidis (50%) indicating its broad spectrum action. VII isolate of V. lecanii recorded maximum mortality (80.8%) of A. craccivora. R. maidis was relatively less susceptible to the three fungi than A. craccivora and A. gossypii. The LC50 of Bb5a for three days old nymphs of A. gossypii was 6.57x105 spores/ml. The LT50 of Bb5a for three days old nymphs of A. gossypii was highest (9.67 days) for the lowest dose of 106 spores/ml, which decreased with increasing concentration. The highest dose 109 spores/ml recorded the lowest LT50 of 1.76 days.Keywords
Aphis craccivora, Aphis gossypii, Beauveria bassiana, Metarhizium anisopiiac, Rhopalosiphum maidi, Verticillium lecanii.Full Text
- Efficacy of Different Isolates of Entomopathogenic Fungi against Brevicoryne brassicae (Linnaeus) at Different Temperatures and Humidities
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Authors
Affiliations
1 Department of Plant Protection, College of Agriculture, University of Shahrood, Shahrood, IR
2 Project Directorate of Biological Control, P.B. No, 2491, H.A. Farm Post, Bellary Road, Bangalore-560 024, IN
1 Department of Plant Protection, College of Agriculture, University of Shahrood, Shahrood, IR
2 Project Directorate of Biological Control, P.B. No, 2491, H.A. Farm Post, Bellary Road, Bangalore-560 024, IN
Source
Journal of Biological Control, Vol 21, No 1 (2007), Pagination: 65-72Abstract
The virulence of twenty-five isolates of entomopathogenic fungi consisting of ten belonging to Beauveria bassiana (Bills.) Vuill., seven to Verticillium lecanii (Zimmerman), five to Metarhizium anisopliae (Mctschinikoff) Sorokin, two to Nomnraea rileyi (Farlow) Samson and one to Paecilomyces fumosorosens (Wize) Brown and Smith originating from a wide range of insect species was investigated in laboratory bioassays on cabbage aphid, Brevicoryne brassicae (Linnaeus) at different regimes of temperature (20, 25 and 30°C) and relative humidity (75, 85, 90 and 95%). All fungal isolates except N. rileyi isolates were pathogenic to the aphid, but in varying degrees. Among three levels of temperature tested, aphid mortality was significantly higher at 25°C than 20 and 30°C. Aphid mortality decreased with decreasing relative humidity. Among all isolates in all combinations of temperature and relative humidity, four isolates of V. lecanii, V.I-l, V.I-2, V.l-6, and V.I-7.showed higher virulence to B. brassicae. In multiple dose bioassays, lowest LT50 was obtained from V.1-7 isolate. The highest virulence of V.1-7 isolate of V. lecanii to B. brassicae suggests that the isolate would be a potential candidate as a microbial control agent for the cabbage aphid.Keywords
Brevicoryne brassicae, Entomopathogenic Fungi, Humidity, Temperature, Verticillium lecanii.Full Text
- Growth Parameters of some Isolates of Entomofungal Pathogens and Production of Dust-Free Spores on Rice Medium
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PDF Views:135
Authors
Affiliations
1 Entomology section, Sugarcane Breeding Institute, Coimbatore-641007, IN
2 Project Directorate of Biological Control (ICAR), Post Bag No. 2491, H. A. Farm Post, Bellary Road Hebbal, Bangalore, 560 024, Karnataka, IN
1 Entomology section, Sugarcane Breeding Institute, Coimbatore-641007, IN
2 Project Directorate of Biological Control (ICAR), Post Bag No. 2491, H. A. Farm Post, Bellary Road Hebbal, Bangalore, 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 19, No 2 (2005), Pagination: 121-128Abstract
Four isolates in each of Beauveria bassiana (Bals.) Vuill, Metarhizium anisopliae (Metschinikoff) Sorokin and Verticillium lecanii (Zimmerman) Viegas, pathogenic to key pests of crops like, coffee berry borer, coconut rhinoceros beetle, cashew stem borer, citrus and coffee green scale etc. were assessed for the colony growth on Potato Dextrose Agar (PDA), biomass and spore production in Potato Dextrose Broth (PDB) and dry spore production in rice grown through di-phasic production system. The isolates Bb5a (B. bassiana), Ma4 (M. anisopliae) and V15 (V. lecanii) were found fast growing with the colony diameter of 6.3, 8.1 and 5.7cm, respectively on PDA after 15 days of incubation. Biomass production in PDB was comparatively higher in shake cultures than in stationary cultures for all isolates except Bb4, BbSa, and V13a. Among the B. bassiana isolates, biomass production in stationary cultures was highest in Bb4 isolate (0.78 g/100ml) and in shake culture in Bb3 isolate (1.02g/100ml). Ma2 in stationary eulture (0.8g/100ml) and Ma3 isolate in shake cultures produced maximum biomass (l.09g/100ml). V1 2a isolate among V. lecanii isolates produced maximum biomass in stationary culture and shake culture (0.77 and 1.03g/100ml, respectively). M. anisopliae isolates formed mycelial pellets of various sizes under shake culture condition in PDB, In two-stage system of mass production, the maximum spore production on rice was observed in BbSa, Ma4 and V15 isolates (96.0, 49.8 and 17.5 x 108 spores g−1) and maximum spores per gram of rice were harvested in Bb5a, Ma2 and V12a isolates (28.00, 45.50 and 15.60 mg g−1). Maximum viable spores per gram of spore dust were recorded in Bb5a, Ma4 and V15 isolates (4.7 x 1010, 2.5 x 1010 and 1.7 x 109g−1). Taking into consideration of quantity of spore dust production and viable spores in the spore dust, Bb5a, Ma2 and V12a were identified as potential isolates for large-scale production of dry conidial powder.Keywords
Beauveria bassiana, Biomass, Metarhizium anisopliae, Radial Growth, Spore Production, Verticillium lecanii.Full Text
- Screening and Selection of Potential Trichoderma Isolates for the Control of Cotton Seed Rot and Damping-off
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Authors
Affiliations
1 Project Directorate of Biological Control (ICAR) P.B. No. 2491, H.A. Farm Post, Bellary Road Bangalore 560024, Karnataka, IN
1 Project Directorate of Biological Control (ICAR) P.B. No. 2491, H.A. Farm Post, Bellary Road Bangalore 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 17, No 2 (2003), Pagination: 161-165Abstract
Out of twelve Trichoderma isolates tested, cotton isolate T. viride (97) exhibited the fastest growth rate and strong antagonism against Rhizoctonia solani in vitro recording 56.2 per cent inhibition in dual culture. All the isolates tested enhanced cotton seed germination when treated with mycelial suspensions of bioagents in vivo. The disease incidence in bioagent treatments ranged between 14.8 to 32.4 per cent, while pathogen treatment recorded 82 per cent. Three isolates have recorded seedling vigour index above 2000, which was much better than fungicide treatment (1471) in blotter tests (Roll Towel Method). T. viride (32) recorded the maximum seed germination (86.4 per cent) and no incidence of post -emergence mortality under green house conditions. The seedling vigour index in bioagent treatments ranged between 1004 and 1866.Keywords
Biocontrol, Cotton, Damping-off, Rhizoctonia solani, Trichoderma.Full Text
- Use of Brewery Waste Amended Spent Malt as Substrate for Mass Production of Trichoderma
Abstract Views :211 |
PDF Views:116
Authors
Affiliations
1 Project Directorate of Biological Control (ICAR) P.B. No. 2491, H.A. Farm Post, Bellary Road Bangalore 560024, Karnataka, IN
1 Project Directorate of Biological Control (ICAR) P.B. No. 2491, H.A. Farm Post, Bellary Road Bangalore 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 17, No 2 (2003), Pagination: 167-170Abstract
Among four different levels of brewers' yeast tested, 3g/100 g of spent malt was found to be optimum for the growth of Trichoderma harzianum in solid-state fermentation. Spent malt brewers' yeast medium supported all the species namely, T. harzianum, T. viride and T. virens. T. harzianum recorded the highest number of viable propagules after twelve days of incubation. All the treatments recorded more number of viable propagules after twelve days of incubation than after seven days of incubation.Keywords
Brewers' Yeast, Solid State, Spent Malt, Trichoderma harzianum.Full Text
- Mass Production of Trichoderma spp. on Spent Meals of Parasitoid Hosts Reared in Laboratory
Abstract Views :195 |
PDF Views:97
Authors
Affiliations
1 Division of Plant Pathology, S. K. University of Agricultural Sciences and Technology, Jammu 180 002, IN
2 Project Directorate of Biological Control (lCAR), H.A. Farm Post, Hebbal, Bellary Road, Bangalore 560024, Karnataka, IN
1 Division of Plant Pathology, S. K. University of Agricultural Sciences and Technology, Jammu 180 002, IN
2 Project Directorate of Biological Control (lCAR), H.A. Farm Post, Hebbal, Bellary Road, Bangalore 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 18, No 1 (2004), Pagination: 91-96Abstract
Suitability of the spent meals of laboratory reared insect parasitoid hosts, namely, Corcyra cephalonica and Sitotroga cerealella for the rnass production of Trichoderma harzianum, T. viride and T. virens was studied. Assessment of optimum moisture level required in C. cephaionica spent rneal for rnass production of Trichoderma spp. was done. Approximately 45 per cent rnoisture yielded rnaximum sporulation of Trichoderma spp. While investigating the utilization of spent meals of C. cephalonica and S. cerealella, it was observed that S. cerealella spent rneal supported maximurn sporulation and viable propagules ill all the three species of Trichoderma and was on par with sorghum as a substrate. However, C. cephalonica meal yielded lowest sporUlation and viable propagules.Keywords
Corcyra, Mass Production, Sitotroga, Trichoderma harzianum, T. Viride, T. Virens.Full Text
- Colonization of Beauveria bassiana (Balsamo) Vuillemin Strains in Maize (Zea mays L.) and their Efficacy against Stem Borer Chilo partellus (Swinhoe)
Abstract Views :267 |
PDF Views:128
Authors
Affiliations
1 ICAR-National Bureau of Agricultural Insect Resources, H.A. Farm Post, Bellary Road, Bengaluru - 560 024, Karnataka, IN
2 ICAR-National Bureau of Agricultural Insect Resources, H.A. Farm Post, Bellary Road, Bengaluru - 560 024, Karnataka, India, IN
1 ICAR-National Bureau of Agricultural Insect Resources, H.A. Farm Post, Bellary Road, Bengaluru - 560 024, Karnataka, IN
2 ICAR-National Bureau of Agricultural Insect Resources, H.A. Farm Post, Bellary Road, Bengaluru - 560 024, Karnataka, India, IN
Source
Journal of Biological Control, Vol 31, No 1 (2017), Pagination: 28-37Abstract
Glasshouse experiments were conducted to study the endophytic ability of six strains of entomopathogenic fungus Beauveria bassiana in maize leaf and stem tissues when applied through foliar sprays of oil formulations. B. bassiana treated and untreated control plants were artificially infested with second instar larvae of Chilo partellus to assess the stem borer damage. All six strains showed varying percent colonization and persistence in stem and leaf tissues. The mean percent colonization ranged from 16.67-46.67 % in older stem tissues and 22.22-52.22 % in young stem tissues and in leaf, it ranged from 25.56-47.78 % in older leaf tissues and 31.11-57.78 % in young leaf tissues. Among six isolates tested, Bb-45 isolate recorded the maximum mean colonization in older stem (46.67 %), older leaf (47.78%) and in young stem (52.22 %). Bb-5a isolate showed maximum mean colonization in young leaf tissues (57.78 %). Significantly lower percentage of dead hearts (2.2-11.1%) and lower stem tunneling (2.7-4.3cm/plant) was observed in B. bassiana treated plants as compared to the untreated control plants (28.86% of dead hearts and 13.41cm/plant stem tunneling).
Keywords
Beauveria Bassiana, Biocontrol, Chilo Partellus, Endophyte, Entomopathogenic Fungus, Maize.Full Text
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- Field Evaluation of Entomopathogenic Fungi against Cabbage Aphid, Brevicoryne brassicae (L.) and their Effect on Coccinellid predator, Coccinella septempunctata (Linnaeus)
Abstract Views :256 |
PDF Views:163
Authors
Affiliations
1 Division of Insect Ecology, ICAR-National Bureau of Agricultural Insect Resources (NBAIR), H. A. Farm Post, Bellary Road, Hebbal, Bengaluru – 560024, Karnataka, IN
2 Division of Insect Ecology, ICAR-National Bureau of Agricultural Insect Resources (NBAIR), H. A. Farm post, Bellary Road, Hebbal, Bengaluru – 560024, Karnataka, IN
1 Division of Insect Ecology, ICAR-National Bureau of Agricultural Insect Resources (NBAIR), H. A. Farm Post, Bellary Road, Hebbal, Bengaluru – 560024, Karnataka, IN
2 Division of Insect Ecology, ICAR-National Bureau of Agricultural Insect Resources (NBAIR), H. A. Farm post, Bellary Road, Hebbal, Bengaluru – 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 31, No 3 (2017), Pagination: 168-171Abstract
The present study was carried out to assess the field efficacy of three isolates of entomopathogenic fungi (EPF) for management of cabbage aphid, Brevicoryne brassicae. All the tested isolates showed suppressive effect on aphid. Two-year pooled data revealed lower incidence of 13.9–17.1 aphids/plant in the EPF treated plots compared to higher incidence of 107.5 aphids/plant in untreated control. Significantly higher yields were recorded in the plots treated with Bb-5a, Ma-4 and Vl-8 isolates (28.0, 27.4 and 27.4 t/ha, respectively) compared to untreated control (22.3 t/ha). The populations of predatory coccinellid and spiders in EPF treated and untreated control plots were found statistically non-significant indicating the safety of entomopathogenic fungi to these predators.Keywords
Beauveria bassiana, Brevicoryne brassicae, Cabbage, Coccinella septempunctata, Entomopathogenic Fungi, Lecanicillium lecanii, Metarhizium anisopliae.Full Text
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- Establishment of Metarhizium anisopliae (Metchnikoff) Sorokin as Endophyte in Maize and Sorghum
Abstract Views :281 |
PDF Views:141
Authors
B. Ramanujam
1,
B. Poornesha
1
Affiliations
1 Division of Insect Ecology, ICAR-National Bureau of Agricultural Insect Resources, H. A. Farm post, Bellary Road, Hebbal, Bengaluru - 560024, Karnataka, IN
1 Division of Insect Ecology, ICAR-National Bureau of Agricultural Insect Resources, H. A. Farm post, Bellary Road, Hebbal, Bengaluru - 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 32, No 3 (2018), Pagination: 165-171Abstract
Glasshouse experiments were conducted to establish an indigenous soil-derived strain of Metarhizium anisopliae (ICAR-NBAIR Ma-35) as endophyte in maize and sorghum by artificial inoculation through foliar spray of conidial suspension. Colonization of M. anisopliae were studied at 15, 30, 45, 60, 75 and 90 Days After Treatment (DAT) in stem and leaf tissues of maize and sorghum by plating technique and PCR method. Metarhizium anisopliae showed variation with regard to the extent of colonization in stem and leaf tissues of maize and sorghum. In maize, colonization was observed at 30DAT in stem and 30-45DAT in leaf. In sorghum, colonization of M. anisopliae was observed during 15-60DAT in stem and 15-75DAT in leaf indicating longer persistence compared to maize. Colonization of M. anisopliae was observed in young growing stem tips and leaves (unsprayed) indicating the internal spread of M. anisopliae in maize and sorghum plants. M. anisopliae was not detected in the untreated stem and leaf tissues of maize and sorghum. The positive results of colonization of M. anisopliae in maize and sorghum stem/leaf tissues observed in plating technique were confirmed by the PCR amplification.Keywords
Endophyte, Entomopathogenic Fungi, Maize, Metarhizium anisopliae, Sorghum.Full Text
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- Evaluation of Entomopathogenic Fungi against Sucking Pests of Bhut Jalakia
Abstract Views :287 |
PDF Views:135
Authors
Affiliations
1 Department of Entomology, Assam Agricultural University, Jorhat - 785013, Assam, IN
2 ICAR-National Bureau of Agricultural Insect Resources, H. A. Farm Post, Bellary Road, Hebbal, Bengaluru - 560024, Karnataka, IN
1 Department of Entomology, Assam Agricultural University, Jorhat - 785013, Assam, IN
2 ICAR-National Bureau of Agricultural Insect Resources, H. A. Farm Post, Bellary Road, Hebbal, Bengaluru - 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 33, No 2 (2019), Pagination: 155-159Abstract
Experiment was conducted to evaluate the efficacy of six different strains of entomopathogenic fungi and one insecticide molecule against sucking pests of Bhut Jalakia during 2014-15 to 2016-17. Bhut Jalakia is one of the hottest chillis in the world, grown extensively in Assam, Nagaland and Manipur. Aphis gossypii Glover, Scirtothrips dorsalis Hood and Bemisia tabaci (Genn.) are the major sucking pests of Bhut Jalakia inflicting damage right from planting to fruiting stages of the crop. Three rounds of biopesticides @ 1 × 108 spores/gm and insecticide (imidacloprid 17.8 SL @ 20 gm a.i./ha) imposed at 3 weeks interval against the pests revealed that imidacloprid 17.8 SL could significantly reduce the mean population of A. gossypii (4.64); S. dorsalis (2.03) and B. tabaci (0.28), closely followed by Beauveria bassiana (NBAIR-Bb-5a) with 7.20, 3.07 and 0.64 per 10 leaves and both the treatments were on par in their efficacy after third spray. The rest of the entomopathogenic fungi were more or less effective in reducing the sucking pests and statistically at par with each other compared to untreated control plot. Highest yield of 52.64 q/ha recorded in imidacloprid treated plot followed by NBAIR strain of Bb-5a with 45.85 q/ha and had no any significant difference from each other. Minimum yield of 26.78 q/ha was obtained in untreated control plot.Keywords
Bhut Jalakia, Biopesticides, Entomopathogenic Fungi, Sucking Pests.Full Text
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- Evaluation of Entomopathogenic Fungi Against Bemisia Tabaci (Gennadius) in Capsicum Under Protected Cultivation
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Authors
Affiliations
1 ICAR-National Bureau of Agricultural Insect Resources (NBAIR), H A Farm Post, Hebbal, Bellary Road, Bengaluru 560024, Karnataka, IN
1 ICAR-National Bureau of Agricultural Insect Resources (NBAIR), H A Farm Post, Hebbal, Bellary Road, Bengaluru 560024, Karnataka, IN
Source
Indian Journal of Entomology, Vol 84, No 1 (2022), Pagination: 160-163Abstract
Effect of indigenous isolates of Beauveria bassiana, Metarhizium anisopliae and Lecanicillum lecanii were tested for two years during 2012 and 2013 on Bemisia tabaci infesting capsicum under protected cultivation. Among the ten isolates tested, NBAIR-Vl8 isolate of L. lecanii, NBAIR-Bb5a and NBAIR-Bb9 isolates of B. bassiana showed significant suppression of Bemisia tabaci (Genn.) with reduction of 73.15, 71.84 and 63.10% respectively. The yields were also superior in these treatments.
Keywords
Bemisia tabaci, entomopathogenic fungi, Capsicum, protected cultivation, Beauveria bassiana, Metarhizium anisopliae, Lecanicillum lecanii, indigenous isolates, NBAIR- V18, NBAIR- Bb5a, NBAIR- Bb9Full Text
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